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Prenatal Gene Therapy
The emerging field of prenatal gene therapy is founded on scientific and technical advances in fetal medicine, molecular biology and gene therapy.This preclinical research subject aims at applying gene therapy during pregnancy for the prevention of human diseases caused by early onset congenital or gestation related conditions.InPrenatal Gene Therapy: Concepts, Methods and Protocols,expert researchers in the field detail many of the protocols which are now commonly used to study gene therapy, fetal medicine and medical ethics. These includedetailed protocols for vector production, for breeding and husbandry of the animal models, for the surgical procedures of gene delivery in large and small animals and forthe methods of gene transfer analysis. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive,Prenatal Gene Therapy:Concepts,Methods and Protocolsseeks to aid scientists in the further study ofprenatal disease and gene therapy projects beyond the scope of fetal medicine.
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