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Der Experimentator: Proteinbiochemie/Proteomics

Der Experimentator: Proteinbiochemie/Proteomics

Brand
Springer Nature
Manufacturer
N/A
Part Number
0
GTIN
9783827423139
Condition
New
Product Description

Die aktualisierte 6. Auflage dieses Buches gibt einen berblick ber die Methoden in Proteinbiochemie und Proteomics. Es zeigt Auswege aus experimentellen Sackgassen und weckt ein Gespr fr das richtige Experiment zur richtigen Zeit. Behandelt werden klassische Verfahren wie Sulenchromatographie, HPLC, Elektrophoresen, Blots, Elisas, Ligandenbindungstests, die Herstellung von Antikrpern, das Solubilisieren von Membranproteinen, die Analyse von Glykoproteinen usw. Einen groen Raum nehmen die modernen Verfahren ein: Massenspektrometrie, Proteomics und thermische Analyse. Neu in der 6. Auflage sind Abschnitte zur Strukturbestimmung und Rekonstitution von Proteinen. Des Weiteren werden neue Tricks zur Proteinbestimmung, Gelfrbung, Blottechnik, Phasentrennung von Membranproteinen, Herstellung von Sucrosegradienten und zur Isolierung von Vesikeln vorgestellt. Auch auf 3D-Gele und Methoden zur Quantifizierung von SH-Gruppen wird eingegangen.

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