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Verfahren der Immunlokalisation
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Der Beitrag von Maria Mulisch bietet einen berblick ber die Techniken der Immunmarkierung fr licht- und elektronenmikroskopische Prparate. Es werden Grundlagen der Eigenschaften, Auswahl, Reinigung und Auswahl von Antikrpern vermittelt und verschiedene Detektionssysteme (Enzyme, Fluorochrome, kolloidales Gold) und Signalverstrkungsmethoden vorgestellt und durch Tabellen ergnzt. Probleme und Fehlerquellen sind tabellarisch zusammengefasst und bieten bersichtlich Lsungsmglichkeiten. Ausgewhlte, detaillierte Anleitungen ergnzen und veranschaulichen den Text.
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