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Bioanalytische Struktur-Funktionsmessungen an Ionenkanlen

Bioanalytische Struktur-Funktionsmessungen an Ionenkanlen

Brand
Springer Nature
Manufacturer
N/A
Part Number
0
GTIN
9783658191443
Condition
New
Product Description

David Schmidt berprft, ob der molekulare Schaltmechanismus, der krzlich in Connexin 26 als rotameres Glu47 mit Fixierung an Lys188 identifiziert wurde, ber Aminosurenaustauschexperimente auf den Kaliumkanal KcsA bertragen werden kann. Die Untersuchung struktureller und funktioneller Einflsse der Punktmutationen erfolgte mittels unterschiedlicher Methoden, darunter die Analyse des Schmelzverhaltens mittels SDS-Gel und spektroskopischer Methoden wie der konfokalen Raman-Mikroskopie oder der Microarray-Technologie. Transporteigenschaften von KcsA und Connexin 26 konnten zudem mittels eines Liposom-Transportassays in Abhngigkeit des pH-Wertes sowie dem Vorhandensein von Inhibitoren untersucht werden.

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