Compare Prices | Create Shopping Lists with Price Drop Alerts | Set Coupons, Promo Codes & Sales Alerts | Get Access to Exclusive Deals and Much More... All in One Place | Read all about it here

Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach

Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach

Brand
Springer Nature
Manufacturer
N/A
Part Number
0
GTIN
9789811073403
Condition
New
Product Description

This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The distinguishing feature of the book is that it covers, in addition to other methods, some of the uncommon but valuable techniques as well. For example, in-gel visualization of enzyme activity, immunoblotting protocols for native (non-denatured) proteins, and proteins resolved by pH-gradient [IEF-isoelectrofocusing], etc. These expedient methods are relevant and vital for the verification of biochemical properties of GAPDH, or similar protein of interest. This work outlines detailed protocols that are essential to investigate classical (cellular) and recently reported extracellular (secretory) isoforms of GAPDH. Precisely, the book covers techniques pertinent to enzymatic and non-enzymatic analysis of GAPDH that include, but not limited to, electrophoretic mobility shift assay (EMSA), two-dimensional (2D)-immunoblotting, immunofluorescence/confocal microscopy, mass spectrometry, ion-exchange and affinity chromatography. Readers will discover the importance of the experimental methods described in the book as they relate to the evaluation of the role and significance of GAPDH. Furthermore, majority of the methods described in the book have also been validated in the authors laboratory, besides other research groups worldwide, underlining the repeatability and reproducibility of the protocols. Each method begins with an abstract and a brief background emphasizing its application and relevance. This will enable the readers to determine the choice of experimental design according to their research objectives. The book explains the methods systematically with ample illustrations to facilitate quick and easy comprehension of the practical knowledge. Although the book is focused on GAPDH, many of the protocols may be adopted to other proteins or enzymes with minimal modifications. Noteworthy, it is unequivocally established that GAPDH is a multifunctional protein involved in several cellular processes of health & disease conditions. Hence, this book will be a valuable practical guide for young researchers, scientists and clinician-scientists.

Available Colors
Available Sizes
1 Offer Price Range: $84.99 - $84.99
BEST PRICE

Single Product Purchase

$84.99
@ Link.springer.com     BUY Now

Price Comparison

Seller Contact Seller List Price On Sale Shipping Best Promo Final Price Volume Discount Financing Availability Seller's Page
BEST PRICE
1 Product Purchase
@ Link.springer.com
Contact Store Call Store Contact Support
$84.99 $84.99

$84.99
See Site In stock Visit Store

Similar Products

Grundlagen der Wirtschafts- und Sozialkybernetik

$44.99

Biomolecules' Conformational Changes Studied by Simulations and Enhanced Sampling

$139.99

Beispiele aus der Baubetriebspraxis

$34.99

Dysphagia Management in Head and Neck Cancers

$249.99

Die rationelle Haushaltfhrung / The New Housekeeping

$59.99

Mutter-Tochter-Beziehungen in der Migration

$59.99

Adsorption and Diffusion

$329.99

Schlingsucht und naturgeme Kauttigkeit

$54.99

Dogmatische Einordnung und prozessuale Konsequenzen einer quantitativen Klageermigung

$89.99

Anesthesiology and Otolaryngology

$169.99

Diskursanalyse und Kritik

$69.99

On the Move to Meaningful Internet Systems. OTM 2017 Conferences

$54.99

Plant Proteomics

$109.00

Real and complex singularities, Oslo 1976

$159.99

Morphologische Befunde bei der bakteriophagen Lyse

$59.99

Dentist Trip

$4.49

Problemorientierte Programmiersprachen

$69.99

The Many Colors of Harpreet Singh

$5.21

Disability and Disaster

$119.99

Nachhaltigkeit und Gerechtigkeit

$79.99

The Ocean is Kind of a Big Deal

$5.24

Berufliche Sozialisation von Bankkaufleuten

$59.99

Blended Learning fr die Unternehmensdigitalisierung

$17.99

Strungen des Wasser-, Elektrolyt- und Sure-Basenhaushaltes

$89.99

Die groe Wahlreform

$69.99

High Quality Connections in der Arbeitswelt

$17.99

Essentials of Medical Ultrasound

$249.00

Liquidittskennziffern und Verschuldungsquote

$59.99

Root Biology

$279.99

Cultural Consultation

$169.99

The Social Construction of Death

$89.99

AIDS Epidemiology

$39.99

The Byzantine City from Heraclius to the Fourth Crusade, 6101204

$119.99

Human Aspects of IT for the Aged Population. Design, Interaction and Technology Acceptance

$119.99

The Other Lepidoptera: Moth Conservation in Australia

$169.99

On the Penitentiary System in the United States and its Application to France

$19.99

Interworking of Wireless LANs and Cellular Networks

$39.99

Natural Resource Management in the Thar Desert Region of Rajasthan

$169.99

Verwaltung, eGovernment und Digitalisierung

$74.99

Advances in Tracer Methodology

$39.99