Nucleic Acid Amplification Technologies: Application to Disease Diagnosis

Name: Nucleic Acid Amplification Technologies: Application to Disease Diagnosis
Brand: Springer Nature
SKU: 9781461275435
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Nucleic Acid Amplification Technologies: Application to Disease Diagnosis

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Springer Nature

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9781461275435

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The polymerase chain reaction (PCR) has proved to be a powerful and versatile tool and has opened new avenues in molecular biol ogy. Alternative nucleic acid amplification techniques, such as the ligase chain reaction (LCR), nucleic acid sequence-based amplifica tion (NASBA), and transcription-mediated amplification (TMA), a variation of NASBA, are also now available. These techniques are all designed to amplify specific nucleic acid sequences in an expo nential manner, thus providing a basis for extremely sensitive diag nostic assays. However, despite the widespread and successful ap plication of genomic amplification techniques in biological research, they have not yet reached the point of routine use in clini cal laboratories. Thus, although the R&D investment in nucleic acid diagnostics is in excess of $250 million annually, clinical ap plications remain relatively modest. One of the principal reasons for this delay in clinical application has been the problem of acci dental contamination of negative clinical specimens with minute amounts of amplified products from a previous positive reaction. Carry-over contamination of amplicons can now be prevented by chemical means or the use of a closed reaction system. However, the current instrumentation is essentially modular in nature, com prising machines that perform the three essential steps of nucleic acid amplification technology: sample preparation, the amplifica tion reaction, and detection of products. Consequently, the test pro cedures are more complicated with somewhat lower sample throughput than the enzyme immunoassays currently performed in clinical laboratories.

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