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Die separate Regulierung zweier Gene mit einfarbigem Licht

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Springer Nature
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9783658081591
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Product Description

Optogenetische Methoden ermglichen die gleichzeitige Manipulation mehrerer Prozesse in Zellen durch den Einsatz von Licht bei verschiedenen Wellenlngen. Da der Einsatz verschiedener Wellenlngen jedoch auch Nachteile mit sich bringt, zeigt Roman S. Iwasaki, wie mit monochromatischen Lichtpulsen zwei verschiedene Prozesse separat reguliert werden knnen, indem ausschlielich die Pulsfrequenz variiert wird. Konkret wird untersucht, wie durch zwei Blaulicht-sensitive Zwei-Komponenten-Systeme die Genexpression in E. coli reversibel induziert werden kann.

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